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t5014 esf921 incest cell culture medium expression system  (Expression Systems Inc)


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    Expression Systems Inc t5014 esf921 incest cell culture medium expression system
    T5014 Esf921 Incest Cell Culture Medium Expression System, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 96/100, based on 817 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t5014+esf921+incest+cell+culture+medium+expression+system/ESF+921+Serum+Free+Insect+Medium/pm41162752-234-132-138
    Average 96 stars, based on 817 article reviews
    t5014 esf921 incest cell culture medium expression system - by Bioz Stars, 2026-09
    96/100 stars

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    Recombinant:

    Article Title: Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptors.
    Article Snippet: .. Reagents and tools table Reagent/ resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94001 F High Five Expression systems Cat#94002 F AD293 ATCC Recombinant DNA pFastBac EP2BRIL This study N/A pFastBac antiBRIL Fab This study N/A pET-21d antiFab Nanobody This study N/A pFastBac EP4 This study N/A pFastBac Fab001 This study N/A pcDNA3.1 EP2 This study N/A pcDNA3.1 EP4 This study N/A Reagent/ resource Reference or source Identifier or catalog number GloSensor plasmid Promega Cat# E2301 Antibodies Anti-FLAG FITCconjugated antibody Sigma Cat# F4049 Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes, and other reagents PF-04418948 TargetMol Cat# T3306 Grapiprant TargetMol Cat# TQ0292 TG6-129 TargetMol Cat# T28958 PGE2 TargetMol Cat# T5014 ESF921 incest cell culture medium Expression system Cat# 96- 001-20 CellfectinTM II Reagent Gibco Cat# 10352100 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glycodiosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 CO2independent medium Gibco Cat# 18045088 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1-5286 COOT www.2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Version 0.9.8.93 12 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/ resource Reference or source Identifier or catalog number UCSF Chimera https://www.cgl.ucsf.edu/chimera/ Version 1.17.3 UCSF Chimera X https://www.cgl.ucsf.edu/chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/scientificsoftware/prism/ Version 8.0.1 AlphaFold2 https://colab.research.google.com/github/ sokrypton/ColabFold/blob/main/ AlphaFold2.ipynb#scrollTo=KK7X9T44pWb7 ColabFold v1.5.5 Other EnVision plate reader EnVision Guava® easyCyte flow cytometer Guava Construction, expression, and purification of EP2-BRILFabBRIL-NbFab complexes The human EP2 receptor construct was engineered to express in its inactive conformation.

    Plasmid Preparation:

    Article Title: Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptors.
    Article Snippet: .. Reagents and tools table Reagent/ resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94001 F High Five Expression systems Cat#94002 F AD293 ATCC Recombinant DNA pFastBac EP2BRIL This study N/A pFastBac antiBRIL Fab This study N/A pET-21d antiFab Nanobody This study N/A pFastBac EP4 This study N/A pFastBac Fab001 This study N/A pcDNA3.1 EP2 This study N/A pcDNA3.1 EP4 This study N/A Reagent/ resource Reference or source Identifier or catalog number GloSensor plasmid Promega Cat# E2301 Antibodies Anti-FLAG FITCconjugated antibody Sigma Cat# F4049 Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes, and other reagents PF-04418948 TargetMol Cat# T3306 Grapiprant TargetMol Cat# TQ0292 TG6-129 TargetMol Cat# T28958 PGE2 TargetMol Cat# T5014 ESF921 incest cell culture medium Expression system Cat# 96- 001-20 CellfectinTM II Reagent Gibco Cat# 10352100 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glycodiosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 CO2independent medium Gibco Cat# 18045088 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1-5286 COOT www.2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Version 0.9.8.93 12 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/ resource Reference or source Identifier or catalog number UCSF Chimera https://www.cgl.ucsf.edu/chimera/ Version 1.17.3 UCSF Chimera X https://www.cgl.ucsf.edu/chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/scientificsoftware/prism/ Version 8.0.1 AlphaFold2 https://colab.research.google.com/github/ sokrypton/ColabFold/blob/main/ AlphaFold2.ipynb#scrollTo=KK7X9T44pWb7 ColabFold v1.5.5 Other EnVision plate reader EnVision Guava® easyCyte flow cytometer Guava Construction, expression, and purification of EP2-BRILFabBRIL-NbFab complexes The human EP2 receptor construct was engineered to express in its inactive conformation.

    Sequencing:

    Article Title: Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptors.
    Article Snippet: .. Reagents and tools table Reagent/ resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94001 F High Five Expression systems Cat#94002 F AD293 ATCC Recombinant DNA pFastBac EP2BRIL This study N/A pFastBac antiBRIL Fab This study N/A pET-21d antiFab Nanobody This study N/A pFastBac EP4 This study N/A pFastBac Fab001 This study N/A pcDNA3.1 EP2 This study N/A pcDNA3.1 EP4 This study N/A Reagent/ resource Reference or source Identifier or catalog number GloSensor plasmid Promega Cat# E2301 Antibodies Anti-FLAG FITCconjugated antibody Sigma Cat# F4049 Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes, and other reagents PF-04418948 TargetMol Cat# T3306 Grapiprant TargetMol Cat# TQ0292 TG6-129 TargetMol Cat# T28958 PGE2 TargetMol Cat# T5014 ESF921 incest cell culture medium Expression system Cat# 96- 001-20 CellfectinTM II Reagent Gibco Cat# 10352100 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glycodiosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 CO2independent medium Gibco Cat# 18045088 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1-5286 COOT www.2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Version 0.9.8.93 12 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/ resource Reference or source Identifier or catalog number UCSF Chimera https://www.cgl.ucsf.edu/chimera/ Version 1.17.3 UCSF Chimera X https://www.cgl.ucsf.edu/chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/scientificsoftware/prism/ Version 8.0.1 AlphaFold2 https://colab.research.google.com/github/ sokrypton/ColabFold/blob/main/ AlphaFold2.ipynb#scrollTo=KK7X9T44pWb7 ColabFold v1.5.5 Other EnVision plate reader EnVision Guava® easyCyte flow cytometer Guava Construction, expression, and purification of EP2-BRILFabBRIL-NbFab complexes The human EP2 receptor construct was engineered to express in its inactive conformation.

    Polymerase Chain Reaction:

    Article Title: Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptors.
    Article Snippet: .. Reagents and tools table Reagent/ resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94001 F High Five Expression systems Cat#94002 F AD293 ATCC Recombinant DNA pFastBac EP2BRIL This study N/A pFastBac antiBRIL Fab This study N/A pET-21d antiFab Nanobody This study N/A pFastBac EP4 This study N/A pFastBac Fab001 This study N/A pcDNA3.1 EP2 This study N/A pcDNA3.1 EP4 This study N/A Reagent/ resource Reference or source Identifier or catalog number GloSensor plasmid Promega Cat# E2301 Antibodies Anti-FLAG FITCconjugated antibody Sigma Cat# F4049 Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes, and other reagents PF-04418948 TargetMol Cat# T3306 Grapiprant TargetMol Cat# TQ0292 TG6-129 TargetMol Cat# T28958 PGE2 TargetMol Cat# T5014 ESF921 incest cell culture medium Expression system Cat# 96- 001-20 CellfectinTM II Reagent Gibco Cat# 10352100 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glycodiosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 CO2independent medium Gibco Cat# 18045088 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1-5286 COOT www.2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Version 0.9.8.93 12 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/ resource Reference or source Identifier or catalog number UCSF Chimera https://www.cgl.ucsf.edu/chimera/ Version 1.17.3 UCSF Chimera X https://www.cgl.ucsf.edu/chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/scientificsoftware/prism/ Version 8.0.1 AlphaFold2 https://colab.research.google.com/github/ sokrypton/ColabFold/blob/main/ AlphaFold2.ipynb#scrollTo=KK7X9T44pWb7 ColabFold v1.5.5 Other EnVision plate reader EnVision Guava® easyCyte flow cytometer Guava Construction, expression, and purification of EP2-BRILFabBRIL-NbFab complexes The human EP2 receptor construct was engineered to express in its inactive conformation.

    Cell Culture:

    Article Title: Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptors.
    Article Snippet: .. Reagents and tools table Reagent/ resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94001 F High Five Expression systems Cat#94002 F AD293 ATCC Recombinant DNA pFastBac EP2BRIL This study N/A pFastBac antiBRIL Fab This study N/A pET-21d antiFab Nanobody This study N/A pFastBac EP4 This study N/A pFastBac Fab001 This study N/A pcDNA3.1 EP2 This study N/A pcDNA3.1 EP4 This study N/A Reagent/ resource Reference or source Identifier or catalog number GloSensor plasmid Promega Cat# E2301 Antibodies Anti-FLAG FITCconjugated antibody Sigma Cat# F4049 Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes, and other reagents PF-04418948 TargetMol Cat# T3306 Grapiprant TargetMol Cat# TQ0292 TG6-129 TargetMol Cat# T28958 PGE2 TargetMol Cat# T5014 ESF921 incest cell culture medium Expression system Cat# 96- 001-20 CellfectinTM II Reagent Gibco Cat# 10352100 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glycodiosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 CO2independent medium Gibco Cat# 18045088 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1-5286 COOT www.2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Version 0.9.8.93 12 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/ resource Reference or source Identifier or catalog number UCSF Chimera https://www.cgl.ucsf.edu/chimera/ Version 1.17.3 UCSF Chimera X https://www.cgl.ucsf.edu/chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/scientificsoftware/prism/ Version 8.0.1 AlphaFold2 https://colab.research.google.com/github/ sokrypton/ColabFold/blob/main/ AlphaFold2.ipynb#scrollTo=KK7X9T44pWb7 ColabFold v1.5.5 Other EnVision plate reader EnVision Guava® easyCyte flow cytometer Guava Construction, expression, and purification of EP2-BRILFabBRIL-NbFab complexes The human EP2 receptor construct was engineered to express in its inactive conformation.

    Protease Inhibitor:

    Article Title: Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptors.
    Article Snippet: .. Reagents and tools table Reagent/ resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94001 F High Five Expression systems Cat#94002 F AD293 ATCC Recombinant DNA pFastBac EP2BRIL This study N/A pFastBac antiBRIL Fab This study N/A pET-21d antiFab Nanobody This study N/A pFastBac EP4 This study N/A pFastBac Fab001 This study N/A pcDNA3.1 EP2 This study N/A pcDNA3.1 EP4 This study N/A Reagent/ resource Reference or source Identifier or catalog number GloSensor plasmid Promega Cat# E2301 Antibodies Anti-FLAG FITCconjugated antibody Sigma Cat# F4049 Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes, and other reagents PF-04418948 TargetMol Cat# T3306 Grapiprant TargetMol Cat# TQ0292 TG6-129 TargetMol Cat# T28958 PGE2 TargetMol Cat# T5014 ESF921 incest cell culture medium Expression system Cat# 96- 001-20 CellfectinTM II Reagent Gibco Cat# 10352100 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glycodiosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 CO2independent medium Gibco Cat# 18045088 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1-5286 COOT www.2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Version 0.9.8.93 12 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/ resource Reference or source Identifier or catalog number UCSF Chimera https://www.cgl.ucsf.edu/chimera/ Version 1.17.3 UCSF Chimera X https://www.cgl.ucsf.edu/chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/scientificsoftware/prism/ Version 8.0.1 AlphaFold2 https://colab.research.google.com/github/ sokrypton/ColabFold/blob/main/ AlphaFold2.ipynb#scrollTo=KK7X9T44pWb7 ColabFold v1.5.5 Other EnVision plate reader EnVision Guava® easyCyte flow cytometer Guava Construction, expression, and purification of EP2-BRILFabBRIL-NbFab complexes The human EP2 receptor construct was engineered to express in its inactive conformation.

    Modification:

    Article Title: Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptors.
    Article Snippet: .. Reagents and tools table Reagent/ resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94001 F High Five Expression systems Cat#94002 F AD293 ATCC Recombinant DNA pFastBac EP2BRIL This study N/A pFastBac antiBRIL Fab This study N/A pET-21d antiFab Nanobody This study N/A pFastBac EP4 This study N/A pFastBac Fab001 This study N/A pcDNA3.1 EP2 This study N/A pcDNA3.1 EP4 This study N/A Reagent/ resource Reference or source Identifier or catalog number GloSensor plasmid Promega Cat# E2301 Antibodies Anti-FLAG FITCconjugated antibody Sigma Cat# F4049 Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes, and other reagents PF-04418948 TargetMol Cat# T3306 Grapiprant TargetMol Cat# TQ0292 TG6-129 TargetMol Cat# T28958 PGE2 TargetMol Cat# T5014 ESF921 incest cell culture medium Expression system Cat# 96- 001-20 CellfectinTM II Reagent Gibco Cat# 10352100 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glycodiosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 CO2independent medium Gibco Cat# 18045088 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1-5286 COOT www.2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Version 0.9.8.93 12 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/ resource Reference or source Identifier or catalog number UCSF Chimera https://www.cgl.ucsf.edu/chimera/ Version 1.17.3 UCSF Chimera X https://www.cgl.ucsf.edu/chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/scientificsoftware/prism/ Version 8.0.1 AlphaFold2 https://colab.research.google.com/github/ sokrypton/ColabFold/blob/main/ AlphaFold2.ipynb#scrollTo=KK7X9T44pWb7 ColabFold v1.5.5 Other EnVision plate reader EnVision Guava® easyCyte flow cytometer Guava Construction, expression, and purification of EP2-BRILFabBRIL-NbFab complexes The human EP2 receptor construct was engineered to express in its inactive conformation.

    Software:

    Article Title: Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptors.
    Article Snippet: .. Reagents and tools table Reagent/ resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94001 F High Five Expression systems Cat#94002 F AD293 ATCC Recombinant DNA pFastBac EP2BRIL This study N/A pFastBac antiBRIL Fab This study N/A pET-21d antiFab Nanobody This study N/A pFastBac EP4 This study N/A pFastBac Fab001 This study N/A pcDNA3.1 EP2 This study N/A pcDNA3.1 EP4 This study N/A Reagent/ resource Reference or source Identifier or catalog number GloSensor plasmid Promega Cat# E2301 Antibodies Anti-FLAG FITCconjugated antibody Sigma Cat# F4049 Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes, and other reagents PF-04418948 TargetMol Cat# T3306 Grapiprant TargetMol Cat# TQ0292 TG6-129 TargetMol Cat# T28958 PGE2 TargetMol Cat# T5014 ESF921 incest cell culture medium Expression system Cat# 96- 001-20 CellfectinTM II Reagent Gibco Cat# 10352100 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glycodiosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 CO2independent medium Gibco Cat# 18045088 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1-5286 COOT www.2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Version 0.9.8.93 12 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/ resource Reference or source Identifier or catalog number UCSF Chimera https://www.cgl.ucsf.edu/chimera/ Version 1.17.3 UCSF Chimera X https://www.cgl.ucsf.edu/chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/scientificsoftware/prism/ Version 8.0.1 AlphaFold2 https://colab.research.google.com/github/ sokrypton/ColabFold/blob/main/ AlphaFold2.ipynb#scrollTo=KK7X9T44pWb7 ColabFold v1.5.5 Other EnVision plate reader EnVision Guava® easyCyte flow cytometer Guava Construction, expression, and purification of EP2-BRILFabBRIL-NbFab complexes The human EP2 receptor construct was engineered to express in its inactive conformation.



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